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Method of Spectrophotometric Microanalysis Based on HRP/PET Self-assembly Film
Abstract： Horseradish peroxidase monolayer was assembled on the surface of PET-CO2￣ substrate. The reaction kinetics of HRP/PET film and H2O2 in micro reactor was studied using improved spectrophotometer. The relative activity of self-assembly HRP/PET film still remains above 80% after storing for 150 days at 4℃. When applied to determination of H2O2 in sample, the recoveries of H2O2 are 96.5%~101.1%. 作 者： Xiu Ming JIANG Zhi Chun CHEN Shao Ming YANG Han Feng LIN Xian Fu LIN 作者單位： Xiu Ming JIANG(Chemistry Department of Zhejiang University, Hangzhou ,310027;Chemistry Department of Zhengzhou Institute of Technology, Zhengzhou, 450052)Zhi Chun CHEN,Shao Ming YANG,Han Feng LIN,Xian Fu LIN(Chemistry Department of Zhejiang University, Hangzhou ,310027)
期 刊： 中國化學(xué)快報(bào)(英文版) ISTICSCI Journal： CHINESE CHEMICAL LETTERS 年，卷(期)： 2004, 15(5) 分類號(hào)： Keywords： Electrostatic self-assembly film horseradish peroxidase (HRP) spectrophotometry atomic force microscope (AFM)