Expression of nitric oxide synthase

Expression of nitric oxide synthase in the developing eye of Zebrafish Danio rerio

Expression of nitric oxide synthase (NOS) in the developing eye of zebrafish was studied by NADPH-diaphorase staining technique. NOS activity was first observed in the optic primordium and the lens placode at 5-somite stage, and remained basically unchanged up to the prim-5 stage. Upon hatching, NOS activity was nearly equally detected in the gangalion cell layer and the photoreceptor layer in the developing retina. However, it began declining in the inner plexiform layer and the inner nuclear layer at this stage. NOS activity disappeared in the lens although the anterior lens epithelium was strongly stained. Two days after hatching, NOS activity was still strong in the photoreceptor layer, but decreased markedly in the gangalion cell layer, the inner plexiform layer and the inner nuclear layer with the retinal patterning. These suggested that nitric oxide (NO), the product of NOS, is not only involved in the modulation of patterning and differentiation of the retinal cells but also in the regulation of proliferation, and differentiation of the lens fibrocytes.

作 者： WANG Yongjun Zhang Shicui M S.Sawant   作者單位： Department of Marine Biology, Ocean Universiy of Qingdao, Qingdao 266003, China  刊 名： 中國海洋湖沼學報（英文版）  ISTIC SCI 英文刊名： CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY  年，卷(期)： 2004 22(4)  分類號： P7  關鍵詞： nitric oxide   eye development   zebrafish  

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