DNA purification and gene typing:Bas

DNA purification and gene typing:Based on multifunctional nanobeads

In this report, a universal protocol for extracting genomic DNA from whole blood, saliva, and bacterial culture by using magnetic nanobeads as solid-phase absorbents was presented. The enrichment of target cells and adsorption of DNA have been functionally integrated onto the surfaces of the carboxyl-modified magnetic nano-beads, and the DNA segments bound on the surface of the beads can be directly used as PCR templates to amplify a target gene. The PCR products were applied to an oligonucleotide array to perform gene typing. The protocol proves to be simple, rapid, biologically and chemically nonhazardous, and promising for the microfabrication of DNA preparation chip.

作 者： XIE Xin ZHANG Xu GAO Huafang ZHANG Huan CHEN Depu CHENG Jing Fei Weiyang   作者單位： XIE Xin(State Key Laboratory of Extraction Separation Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China;National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China;These authors contributed)

ZHANG Xu(Faculty of Science, Sichuan Agricultural University, Ya'an 625014, China;National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China;These authors contributed equally to this work)

GAO Huafang(Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China;These authors contributed equally to this work)

ZHANG Huan(Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge MA 02139, USA)

CHEN Depu(Department of Chemistry, Tsinghua University, Beijing 100084, China)

CHENG Jing(Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing 100084, China;National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China)

Fei Weiyang(State Key Laboratory of Extraction Separation Engineering, Department of Chemical Engineering, Tsinghua University, Beijing 100084, China) 

刊 名： 科學通報(英文版)  SCI 英文刊名： CHINESE SCIENCE BULLETIN  年，卷(期)： 2004 49(9)  分類號： Q3  關鍵詞： multifunctional nanobeads   miniprep   genomic DNA   HLA typing   microchip  

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